pFD666


Contributed Complete Sequence Link :
Vector IG Incomplete Sequence Link :
General : cosmid ds-DNA 5250 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli)(Actinomycetes)(Streptomyces lividans)(Saccharopolyspora erythraea)
Suppliers : (ATCC)
Misc.Comments : The order of the major features in this plasmid is:rrnC terminator - SP6 - SphI/MCS/HindIII - T7 - trpA terminator - neo - synthetic promoter -pMB1 ori - cos - M13 ori - pJV1 ori. [1] Cosmid shuttle vector permitting production of ssDNA and containing promoters for in vitro RNA synthesis. Terminators flanking the polylinker protect the vector from transcripts originating in cloned inserts. [1] The ori of pJV1 from Streptomyces phaeochromogenes is compatible with plasmids derived from pIJ101. This vector can be used for complementation studies using multiple cloned genes in one host. [1] M13K07 helper phage improves the yield of ssDNA in E.coli with this vector. M13mp18 may also be used. [1] The neo promoter was replaced by a synthetic promoter recognized by both hosts. [1] Restriction digests of the clone give the following sizes (kb): BamHI--5.4; PstI--5.4; PvuII--3.7, 1.7; SacI--4.5, 0.9. (ATCC staff) Medium is 1236 LB plus kanamycin.
Parents : (pFDNEO)(pJV1)
Siblings : ()
Descendents : ()


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