pfd-Tn5


Vector IG Sequence Link :
General : plasmid ds-DNA 8400 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli)(E.coli C600-2Sm)
Suppliers : (ATCC)
Misc.Comments : Deposited by: Klaus Geider Suicide plasmid for transposon-mediated or site-directed mutagenesis of Erwinia amylovora and Escherichia coli after electroporation. [1] Suggested conditions for electroporation in these bacterial strains: E. coli C600Sm, fields strength 20 kV/cm, pulse length 5 to 12 msec; Erwinia amylovora Ea7/74Sm, 25 kV/cm, 5 msec; E. amylovora Ea1/79Sm, 12.5 kV/cm, 17.5 msec. [1] To propagate the plasmid, the host must provide the bacteriophage fd gene 2 product. [1] The order of the major features in this plasmid is: EcoRI - ampR - fd ori - EcoRI - Tn5/kanR (6.5 kb). [1] Restriction digests of the clone give the following sizes (kb): EcoRI--6.5, 2.0; BamHI--4.4, 4.0; HindIII--5.0, 3.4; PstI--2.45 (doublet); 1.5, 0.96, 0.84. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()


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