Vector IG Sequence Link :
General : plasmid ds-DNA 4000 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli)(mouse fibroblast cells)
Suppliers : ()
Misc.Comments : A recombinant plasmid expressed inserted DNA sequences coding for prokaryotic methotrexate-resistant dihydrofolate reductase of unspecified origin, using an SV40 early promoter and polyadenylation signals from the rabbit beta-globin gene. The DHFR insert begins at base 4. The plasmids were grown in mouse fibroblasts and produced a chimeric mRNA coding for the prokaryotic enzyme. The mouse fibroblasts became Mtx resistant. [1] also reports a DHFR sequence in plasmid pFE373. NCBI gi: 208994
Parents : ()
Siblings : (pFE373)
Descendents : ()


Return to Vector Homepage