pFL34


Vector IG Sequence Link :
General : plasmid ds-DNA 3799 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(S.cerevisiae)(E.coli)(Saccharomyces)
Suppliers : (ATCC)
Misc.Comments : pFL34 was constructed from pUC19 plasmid where two alu I sites were modified. The site 629 was replaced by a BglII linker and the site 747 by a ClaI site. The yeast selectable marker has been cloned in the BglII site. The order of the major features in this plasmid is:EcoRI/MCS/HindIII - URA3 - pMB1 ori - ampR. YI-type shuttle integration vector permitting visual detection of recombinants based on alpha complementation. Restriction digests of the clone give the following sizes (kb): BamHI--3.9; BglII--2.8, 1.1; ClaI--3.9. (ATCC staff) Medium is 1227 LB plus ampicillin. NCBI gi: 311965
Parents : (pUC19)(yeast 2-micron plasmid)
Siblings : ()
Descendents : ()

NCBI ENTREZ Link :


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