pFL45S


Vector IG Sequence Link :
General : plasmid ds-DNA 4063 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(S.cerevisiae)(E.coli)(Saccharomyces)
Suppliers : (ATCC)
Misc.Comments : pFL45S was constructed from pUC19 plasmid where two alu I sites were modified. The site 629 was replaced by a BglII linker and the site 747 by a ClaI site. The yeast selectable marker has been cloned in the BglII site and the 2 micron 0.5 Kb FspI fragment containing its replication origin has been cloned at the ClaI site. The order of the major features in this plasmid is:EcoRI/MCS/HindIII - TRP1 - 2 micron ori - pMB1 ori - ampR. YE-type high copy number shuttle vector permitting visual detection of recombinants based on alpha complementation. The loss per generation (percentage having lost all the plasmids after 20 h of non-selective growth) is 11.0. Restriction digests of the clone give the following sizes (kb): BamHI--4.2; BglII--3.4, 0.8; ClaI--3.7, 0.5. (ATCC staff) Medium is 1227 LB plus ampicillin. NCBI gi: 397133
Parents : (pUC19)(yeast 2-micron plasmid)
Siblings : ()
Descendents : ()

NCBI ENTREZ Link :


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