Vector IG Sequence Link :
General : plasmid ds-DNA 3627 BP
Functions : (expression)(fusion)(cloning directional)
Selection : (color blue/white)
Copy Number :
Hosts : (E.coli)(E.coli HB101)
Suppliers : ()
Misc.Comments : Deposited by: Mark E. Brandt Restriction digests of the plasmid give the following sizes (kb): BamHI--3.574, 0.053; BglI--1.843, 1.784; PstI--2.072, 1.555; PvuI--2.063, 1.564; PvuII--2.972, 0.655; SacI--3.257, 0.37. (GenBank/EMBL record) Expression vector permitting the production of a fusion protein that can be trimmed by cleavage with the coagulation factor Xa protease. A modified lacZ' allows blue/white screening of colonies. [1] Especially useful for the expression of PCR products. [1] Inserts can be cloned using the 5' StuI site and one other MCS restriction site matching the 3' end of the insert. The vector supplies the AUG initiator codon. (personal communication) The insert to be cloned should have a phosphorylated 5' blunt end with the first base corresponding to the first base of the first codon. (personal communication) The order of the major features of the plasmid is: pMB1 ori - ampR - lambdaPL promoter - lambda cII - factor Xa recognition sequence - StuI/MCS/HindIII/modified lacZ'. [1] Restriction digests of the clone give the following sizes (kb): BglI--1.8 (doublet); EcoRI--3.6; PstI--2.1, 1.55. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : (lambda)(pBR322)
Siblings : ()
Descendents : (pcIIhFR also from pUC/AR)(phFR also from pdeltablue)(phFR18+ from pUC/AR18+)


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