pGCAT-A


Vector IG Sequence Link :
General : plasmid ds-DNA 4556 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(vertebrate cells)(E.coli)
Suppliers : (ATCC)
Misc.Comments : Restriction digests of the clone give the following sizes (kb): PvuII--4.3, 0.2; HincII--3.0, 1.5; EcoRI--4.5. (ATCC staff) The polylinker is in opposite orientation in pGCAT-A (ATCC 37640) and pGCAT-C (ATCC 37641). The sequence at the junction of the multiple cloning site and CAT is GAATTAGCTT and at the junction of SV40 and SP6 is GGATCAATTC. (personal communication) The BamHI/HindIII fragment of pSB1 containing CAT was cloned into the EcoRI site of pGEM-4 (all ends blunt). Contains the SV40 polyadenylation site and donor and acceptor sites of SV40 small t antigen intron. A plasmid shuttle vector for investigating promoter and/or enhancer elements. (ATCC staff) Has a polylinker upstream of the reporter CAT gene and demonstrates low basal CAT expression in eukaryotic cells. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
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