Vector IG Sequence Link :
General : plasmid ds-DNA 4552 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(vertebrate cells)(E.coli)
Suppliers : (ATCC)
Misc.Comments : Restriction digests of the clone give the following sizes (kb): PvuII--3.0, 1.5; HincII--3.0, 1.5; EcoRI--4.25, 0.3. (ATCC staff) The polylinker is in opposite orientation in pGCAT-A (ATCC 37640) and pGCAT-C (ATCC 37641). The sequence at the junction of the multiple cloning site and CAT is GAATTCGAGC and at the junction of SV40 and SP6 is GGATCAGCTT. (personal communication) BamHI/HindIII fragment of pSB1 (CAT) was cloned into the HindIII site of pGEM-4. 2 hrs after ligation began the remaining BamHI and HindIII sites were filled in. Contains SV40 polyadenylation site and donor and acceptor sites of SV40 small-t intron. A plasmid shuttle vector for investigating promoter and/or enhancer elements. Has a polylinker upstream of reporter CAT gene, SP6 and T7 promoters for in vitro RNA synthesis and low basal CAT expression in eukaryotic cells. Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()

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