Vector IG Sequence Link :
General : plasmid ds-DNA 4932 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(E.coli)(E.coli TG1)(E.coli JM101)
Suppliers : (ATCC)
Misc.Comments : Expression vector permitting production of a fusion protein. (ATCC staff) The glutathione S-transferase (GST) fusion protein can be purified by glutathione affinity chromatography, and the desired polypeptide released from the fusion product by thrombin. [2] Constructed from pGEX-2T by inserting an oligonucleotide at the EcoRI site which encodes the glycine kinker and additional restriction sites to facilitate cloning in all reading frames. [2] The order of the major features in this plasmid are: Ptac - GST - thrombin cleavage site - BamHI site - SmaI site - glycine kinker - multiple cloning site - ampR - pBR322 ori - lacIq. [2] Restriction digests of the clone give the following sizes (kb): HindIII--5.0; BamHI--5.0; EcoRI--5.0; XbaI--5.0. (ATCC staff) Medium is 1227 LB plus ampicillin. NCBI gi: 208304
Parents : ()
Siblings : ()
Descendents : ()


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