pGEX-KN


Vector IG Sequence Link :
General : plasmid ds-DNA 4966 BP
Functions : (cloning)(expression)(affinity)
Selection : ()
Copy Number :
Hosts : (E.coli)(E.coli HB101)(E.coli JM101)(E.coli TG1)(E.coli BL21)
Suppliers : (ATCC)
Misc.Comments : Deposited by: David J. Hakes, Jack E. Dixon The glutathione S-transferase (GST) fusion protein can be purified by glutathione affinity chromatography, and the desired polypeptide released from the fusion product by thrombin. [1] Expression vector for rapid purification of fusion proteins that contain no amino terminal extensions after thrombin cleavage. The amino acid after the initiator methionine must be charged. [1] Cloning into this vector requires amplification of the gene using oligonucleotides prepared as in the reference and encoding the first 4 amino acids of a thrombin recognition sequence. [1] Constructed from pGEX-1 by inserting an oligonucleotide at the BamHI site which encodes the glycine kinker and a NotI site. [1] The order of the major features in this plasmid is: pMB1 ori - lacIV - lacZ - tac promoter - GST - glycine kinker - NotI/MCS/EcoRI - ampR. [1] Restriction digests of the clone give the following sizes (kb): NotI--5.0; EcoRI/PstI--4.0, 1.0; BamHI/EcoRV--3.2, 1.8. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : (pGEX-1)
Siblings : (pGEX-KT)(pGEX-KG)
Descendents : (pGEX-KN-PTPU323)(pGEX-KN-LAMBDA-PP)(pGEX-KN-YERSINIA-PP)


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