Vector IG Sequence Link :
General : plasmid ds-DNA 4978 BP
Functions : (cloning)(expression)(affinity)
Selection : ()
Copy Number :
Hosts : (E.coli)(E.coli HB101)(E.coli JM101)(E.coli TG1)(E.coli BL21)
Suppliers : (ATCC)
Misc.Comments : Deposited by: David J. Hakes, Jack E. Dixon The glutathione S-transferase (GST) fusion protein can be purified by glutathione affinity chromatography, and the desired polypeptide released from the fusion product by thrombin. [1] Expression vector for rapid purification of fusion proteins and release of proteins with fewer extraneous N-terminal amino acids. [1] The resulting protein contains two additional amino acids at the amino terminus (Gly-Ser). [1] Constructed from pGEX-1 by inserting an oligonucleotide at the BamHI site which encodes the glycine kinker and thrombin recognition site to enhance cleavage of the fusion protein. [1] The order of the major features in the plasmid is: pMB1 ori - lacIq - lacZ - tac promoter - GST - glycine kinker - thrombin cleavage site - BamHI/MCS/EcoRI - ampR. [1] Restriction digests of the clone give the following sizes (kb): BamHI--5.0; EcoRI/PstI--4.0, 1.0; EcoRI/EcoRV--3.2, 1.8. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : (pGEX-1)
Siblings : (pGEX-KN)(pGEX-KG)

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