pGPTV-BAR


Vector IG Sequence Link :
General : plasmid ds-DNA 13400 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli)(E.coli HB101)(Agrobacterium tumefaciens)(higher plants)(Agrobacterium tumefaciens LBA4404)
Suppliers : (ATCC)
Misc.Comments : Deposited by: Robert Masterson, D. Becker Binary vector with plant selectable marker near the T-DNA left border and a glucuronidase (uidA) reporter gene. The marker and uidA are divergently transcribed, and can be easily removed or replaced. [1] The order of the major features of this plasmid is: EcoRI - pAnos - SstI - uidA - SmaI - XbaI - HindIII - Pnos - BglII - bar - BglII - BamHI - pAg7- T-DNA - BglII - RK2 ori - kanR. [1] Vector contains the following restriction sites (approximate kb from the HindIII site): BamHI--1.2; EcoRI--11.2; SstI--11.5. [1] Restriction digests of the clone give the following sizes (kb): EcoRI--13.0; BglII--8.4, 3.1, 1.4, 0.56; BamHI--13.0. (ATCC staff) Medium is 1236 LB plus kanamycin.
Parents : ()
Siblings : ()
Descendents : ()


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