pGRE5-1


Vector IG Sequence Link :
General : plasmid ds-DNA 4000 BP
Functions : (cloning)(expression eukaryote)
Selection : ()
Copy Number :
Hosts : (E.coli)(eukaryote cells)
Suppliers : ()
Misc.Comments : Plasmid to be cotransfected with plasmids encoding steroid receptors such as pRShGR (ATCC 67200) and pRShMR (ATCC 67201) to provide a reporter gene for hormone-dependent transcriptional activation. [3] Contains the LTR from mouse mammary tumor virus (nt -142 to nt -109), sequences encoding chloramphenicol acetyltransferase, and SV40 splicing sequences. [3] Restriction digests of the clone give the following sizes (kb): EcoRI--4.2, 1.8; PvuII--5.95, 0.3; BglI--4.8, 1.2; HindIII--3.55, 2.45; PstI--6.0. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : (pGRE5-2)(pGRE5CAT)(pGRE5-1EBV)(pGRE5-2EBV)(pGRE5CATEBV)
Descendents : ()


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