Vector IG Sequence Link :
General : plasmid ds-DNA 9500 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli)(E.coli HB101)(vertebrate cells)
Suppliers : (ATCC)
Misc.Comments : Deposited by: Alexandra L. Joyner Gene trap vector designed to identify and mutate endogenous mammalian genes by integration and production of a fusion protein with the lacZ reporter gene. Can also be used to monitor endogenous gene expression. [1] Identified cDNA sequences spanning the lacZ splice junction can be cloned using the published sequence of the En-2 splice acceptor and the rapid amplification of cDNA ends (RACE) protocol. [1] Derived from pGT4.5 by replacing the En-2 polyadenylation signal with an SV40 polyadenylation signal. (personal communication) The order of the major features in this plasmid is: pUC18 - HindIII - En-2 intron - splice acceptor - En-2 homeobox exon - lacZ - SV40 polyadenylation - human beta-actin promoter - neoR - SV40 polyadenylation. [2] Restriction digests of the clone give the following sizes (kb): BamHI--3.8, 3.3, 1.8, 0.45, 0.2; HindIII--9.5; XbaI--4.6, 3.1, 1.0, 0.8. (ATCC staff) Medium is 1227 LB plus ampicillin. NCBI gi: 403021
Parents : ()
Siblings : ()
Descendents : ()


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