Vector IG Sequence Link :
General : cosmid ds-DNA 6524 BP
Functions : (cloning 30000 to 40000 bp)(in vitro packaging)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(E.coli LE392)(E.coli JM83)(E.coli 5K)(E.coli)
Suppliers : (BRL)(ATCC)
Misc.Comments : Boehringer size is 6524 bp. BRL size is 6523 bp; it is missing the c at 6481. ATCC size is 6430 bp. The lambda cohesive end site (cos region) is needed for packaging into pre-mature phage heads. pBR322 was partially digested with Sau3A and ligated with an 0.65 BglII lambda fragment, generating a BglII site (pBR322 positions 1459-1666 deleted). A 1.65 kb BglII fragment from Charon 4A was ligated into the BglII site. [1] A general purpose cosmid vector derived from pBR322. [1] Medium is 1227 LB plus ampicillin. old Boehringer vector.
Parents : (pBR322)(lambda cos)
Siblings : ()
Descendents : (pV34)


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