pHG165


Vector IG Sequence Link :
General : plasmid ds-DNA 3375 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli JM101)(E.coli)(E.coli JM series)
Suppliers : (ATCC)
Misc.Comments : Created by Moore, July 1995, under contract with NCBI. Enzymes that cut only once (not including polylinker): AatII, AvaI, BalI, ScaI. The HaeII sites flanking lac in pUC8 were changed to HpaI sites. pBR322 was modified by removing PstI & HincII sites in ampR gene and changing the EcoRI to HpaI. A HpaI cassette with lac & MCS was cloned at the HpaI of the modified pBR322. pHG165 enables selection for inserts on MacConkey agar. The lac gene and multiple cloning sites are easily removed with a HpaI digest. Plasmid vector with the lac gene and polylinker of pUC8 but with copy number control of pBR322. Restriction digests of the clone give the following sizes (kb): HindIII--3.5; HpaI--3.0, about 0.5. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : (pBR322)(pUC8)
Siblings : ()
Descendents : ()


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