pHisGal


Vector IG Sequence Link :
General : phagemid ds-DNA 3910 BP
Functions : (cloning)(fusion)
Selection : ()
Copy Number :
Hosts : (E.coli)(E.coli XL1-Blue)
Suppliers : (ATCC)
Misc.Comments : Restriction digests of the clone give the following sizes (kb): HindIII--4.0; PvuI--4.0; XbaI--4.0. (ATCC staff) Expression vector for production of Gal4 fusion proteins, allowing protein purification by affinity chromatography through a nickel nitrilotriacetic acid (Ni-NTA) column. For expression of cloned inserts, the medium should contain 10 uM zinc chloride and expression can be induced using IPTG. [1] Plasmid contains the following restriction sites (approximate kb from the BamHI site): XbaI--1.51; PvuI--3.15. [1] Growth: LB plus ampicillin (ATCC medium number 1227) 37C Deposited by: Schmitz M.K.
Parents : ()
Siblings : ()
Descendents : ()


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