pHPS9R


Vector IG Sequence Link :
General : plasmid ds-DNA 4100 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (Bacillus subtilis 6GM15)(Bacillus subtilis)(E.coli)
Suppliers : (ATCC)
Misc.Comments : Recommended for pHPS9 (ATCC 37816, 37817) to permit alpha-complementation and plasmid marker rescue transformation for transformation of competent cells with plasmid monomers. pHPS9R serves as the helper plasmid. Differs from pHPS9 (ATCC 37816, 37817) by partial removal of chloramphenicol resistance sequence (downstream of the NcoI site) and removal of the P59 promoter and sequences encoding the cat86::lacZalpha fusion. Plasmid was not verified by restriction analysis. Strain was checked for the following phenotypes: showed growth on LB + erythromycin (150 ug/ml), white colonies on Xgal +IPTG, white colonies on Xgal + IPTG + erythromycin (150 ug/ml). (ATCC staff) Medium is -1 1065 plus erythromycin (150 ug/ml).
Parents : ()
Siblings : ()
Descendents : ()


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