Vector IG Sequence Link :
General : plasmid ds-DNA 4870 BP
Functions : (shuttle)(transformation)
Selection : ()
Copy Number :
Hosts : (E.coli)(B.subtilis)(E.faecalis strain DS-5)(S.faecalis)
Suppliers : ()
Misc.Comments : PHY300PLK is one of the smallest hybrids of a new series of chimeric plasmids using the parental plasmids, pACYC177 of E.coli and pAM-alpha of Streptococcus faecalis. This shuttle vector for E.coli and B.subtilis contains an RNA primer gene for ori-177; the R-Tc gene; the R-Ap gene; Rep-alpha-1 gene, which is the plasmid replication gene; two replication origins, one for E.coli and the other for B. subtilis; and a polylinker. Twenty-three unique recognition restriction endonuclease sites have been found in pHY300PLK.
Parents : (pACYC177)(pAMalpha)
Siblings : (pHY320PLK)
Descendents : ()


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