Vector IG Sequence Link :
General : plasmid ds-DNA 8800 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(Saccharomyces cerevisiae)(E.coli)(Saccharomyces cerevisiae YPH485)(Saccharomyces cerevisiae YPH494)
Suppliers : (ATCC)
Misc.Comments : YE-type shuttle vector permitting FLP-driven high copy number. (personal communication) In S. cerevisiae, requires a host expressing FLP such as YPH485 or YPH494, or a host modified by pFV17 (ATCC 77170). (personal communication) When propagated in a strain carrying an integrated copy of GAL10-FLP, the copy number is 10-20 without induction and 200-400 when induced by galactose. The order of the major features in this plasmid is:REP3 - D - REP1 - REP2 - kanR - MCS (NotI - EcoRI/NotI) - URA3. Constructed by inserting a BalI/EcoRV fragment of the 2 micron circle (interrupting FLP) into the SmaI site of pHSS6 which had been modified by the addition of a URA3 gene. Restriction digests of the clone give the following sizes (kb): BamHI--8.8; HindIII--4.3, 3.3, 1.2; NotI--8.8; ClaI--8.8. (ATCC staff) Medium is 1236 LB plus kanamycin.
Parents : ()
Siblings : ()
Descendents : ()

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