pIC20R


Vector IG Sequence Link :
General : plasmid ds-DNA 2716 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli TB1)(E.coli)
Suppliers : (ATCC)
Misc.Comments : These data and their annotation were supplied to GenBank by Will Gilbert under the auspices of the GenBank Currator Program. Assembled from pUC19 and pIC7 by F. Pfeiffer For construction of pIC7, a synthetic oligonucleotide has been used to replace the pUC8 polylinker and thus to construct a new cloning vector with a different polylinker. The other pIC-vectors are based on this new pIC7 polylinker, which was combined with the existing pUC9 and pUC19 polylinkers in the following arrangements: pIC19 and pICEM19 vectors: pIC19R: EcoRI- Poly (pIC7) -HindIII- Poly (pUC9) -EcoRI pIC19H: HindIII- Poly (pUC9) -EcoRI - Poly (pIC7) -HindIII pIC20-vectors: pIC20R: EcoRI- Poly (pIC7) -HindIII- Poly(pUC19) -EcoRI pIC20H: HindIII- Poly(pUC19) -EcoRI - Poly (pIC7) -HindIII To produce greater versatility of insertional inactivation of beta-galactosidase activity for subcloning and sequencing, a chemically synthesized oligonucleotide, specifying nine restriction sites including BglII, XhoI, NruI, ClaI, SacI and EcorV in various configurations with existing polylinkers, was created. These improved polylinkers were inserted into plasmids for routine cloning of ds-DNA and into chimeric phage/plasmids for biological production of ss-DNA. The most versatile polyrecognition pattern specifies 17 restriction sites in the beta-galactosidase alpha-complementing gene fragment. Clone pIC7 was used to produce all the other polylinker-carrying vectors. Streak to xgal + amp plate and pick a blue colony. A general purpose plasmid vector with a polylinker containing 17 sites in lacZ'. Medium is 1227 LB plus ampicillin.
Parents : (pIC7)(pUC19)(pICEM20H)
Siblings : ()
Descendents : ()

NCBI ENTREZ Link :


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