pIN III ompA3
Vector DB Sequence Link :
General :Secretion-expression plasmid 7025 BP
Functions : expression of proteins in E. coli
Selection : Amp, ompA signal peptide, lacI gene
Copy Number :
Hosts : E.coli
Misc.Comments : 7025 bp utilizes
lpp-lac fusion promoter for IPTG-inducible expression
Attaches ompA signal peptide to N-terminus of cloned protein to promote
secretion of protein into periplasm if protein is membrane-bound it
will anchor in the plasma membrane. Contains lacI repressor gene.
Encodes ampicillin resistance, and uses pBR origin of replication.
A high copy number vector (pIN4) is also available.
Reference: Ghrayeb et al., EMBO J. 3: 2437-2442, 1984.
Sequence determined and contributed to GenBank and VectorDB by Todd D. Porter, University of Kentucky,
College of Pharmacy, Lexington, KY (firstname.lastname@example.org)
NCBI ENTREZ Link :
Return to Vector Homepage