Vector IG Sequence Link :
General : plasmid ds-DNA 3700 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(vertebrate cells)(E.coli)(E.coli DH5)
Suppliers : (ATCC)
Misc.Comments : Restriction digests of the clone give the following sizes (kb): EcoRI--3.7; HindIII--3.7; BamHI--3.7; SmaI--3.7; PvuI--2.2, 1.5. (ATCC staff) Constructed from pBR322 (2066-4286, bla, ori), SV40 (4038/4648, t antigen IVS), SV40 (2461-2704, T antigen polyadenylation site), and MMTV LTR (-360 to +150, glucocorticoid regulatory sequences, cap site). (personal communication) Demonstrates expression inducible by dexamethasone. A mammalian shuttle vector for expression of a sequence cloned into the polylinker site under the control of the MMTV LTR promoter. (ATCC staff) Medium is 1315 1065 plus ampicillin (100 ug/ul).
Parents : ()
Siblings : ()
Descendents : ()

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