pJA73


Vector IG Sequence Link :
General : plasmid ds-DNA 5007 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(Saccharomyces cerevisiae)(E.coli)
Suppliers : (ATCC)
Misc.Comments : YC-type shuttle vector that can be used with the Tn3-based transposition system developed in Heffron's laboratory. Permits visual detection of recombinants by the lacZalpha complementation system. (personal communication) The order of the major features in this plasmid is:lacZ'/MCS - URA3 - CEN6 - ARS H4 - kanR - pMB1 ori. (personal communication) Restriction digests of the clone give the following sizes (kb): PstI--1.9, 1.9, 1.4; EcoRV--4.2, 1.1; EcoRI--5.3; KpnI--5.3. (ATCC staff) Medium is 1236 LB plus kanamycin.
Parents : ()
Siblings : ()
Descendents : ()


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