pJB8


Vector IG Sequence Link :
General : cosmid ds-DNA 5464 BP
Functions : (cloning 30000 to 40000 bp)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(E.coli)
Suppliers : (ATCC)
Misc.Comments : Restriction digests analyzed on agarose gels give the following sizes (kb): HindIII--5.4; BamHI--5.4; PstI/HindIII--4.6, 0.8; PstI/SalI--3.3, 2.3. (ATCC staff) A cosmid vector containing a single cos site. [2] Cloning into the BamHI or EcoRI site involves 2 preparations of dephosphorylated vector, one linearized with HindIII, the other linearized with SalI. [2] An equimolar ratio of right-hand and left-hand cos containing vector fragments, digested with BamHI or EcoRI can be ligated to appropriately digested and dephosphorylated inserts. [2] Only molecules with 2 cos sites separated by 37-50 kb can be packaged. [2] Medium is 1227 LB plus ampicillin.
Parents : (Charon 4)(pAT153)
Siblings : ()
Descendents : ()

NCBI ENTREZ Link :


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