pJEL144


Vector IG Sequence Link :
General : plasmid ds-DNA 12700 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli CSH50)(E.coli)
Suppliers : (ATCC)
Misc.Comments : Terminator-testing plasmid. Termination signal-containing sequences cloned into the BamHI site may reduce expression of beta-galactosidase activity regulated by the deoP2 promoter. Below 37C, there is one copy per genome equivalent stabilized by the parB locus. At 42C replication is uncontrolled because induction of the cI857-regulated PR promoter permits expression of the copB, copA, and repA genes. Restriction digests of the clone give the following sizes (kb): BamHI--13.6; EcoRI--13.6. (ATCC staff) Constructed by inserting a 0.22 kb EcoRI/BamHI fragment containing the deoP2 regulatory region and the first 16 codons of deoC from pJEL134 into pJEL170. Transcription from deoP2 is negatively regulated by the DeoR and CytR repressors and depends on the cAMP/CRP complex. Expression of beta-galactosidase from this vector can be increased by the use of hosts containing repressor mutations such as S0929 (cytR), S0930 (deoR) and S0931 (cytR, deoR). Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
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