Vector IG Sequence Link :
General : plasmid ds-DNA 12500 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli CSH50)(E.coli)
Suppliers : (ATCC)
Misc.Comments : Restriction digests of the clone give the following sizes (kb): BamHI--13.0; EcoRI--13.0. (ATCC staff) Below 37C, there is one copy per genome equivalent stabilized by the parB locus. At 42C replication is uncontrolled because induction of the cI857-regulated PR promoter permits expression of the copB, copA, and repA genes. A promoter-cloning vector using increased expression of beta-galactosidase as the reporter. Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()


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