Vector IG Sequence Link :
General : plasmid ds-DNA 5950 BP
Functions : (cloning)(expression)(fusion)(shuttle)
Selection : (color)
Copy Number :
Hosts : (E.coli)(Clostridium perfringens)
Suppliers : (ATCC)
Misc.Comments : Restriction digests of the clone give the following sizes (kb): BglI--3.85, 2.1; PvuI--5.95; SmaI--5.95. (ATCC staff) The standard reverse sequencing primer is not useful in this vector due to the duplication of the 181 bp region between pUC18 sites PvuII and EcoRI. (Plasmid 27: 207-219, 1992) Shuttle expression vector derived from pJIR418 (ATCC 77387) permitting expression of antibiotic resistance in both hosts. The erythromycin resistance gene (ermBP), expressed in both C. perfringens and E. coli, was derived from C. perfringens and is inactivated by insertions at the ScaI site. The vector contains the following restriction sites (approximate kb from the EcoRI site): BglI--0.20, 2.50; NdeI--0.27, 2.09; PvuI--0.17; PvuII--0.14, 5.78; ScaI--4.45; SmaI--4.98. The order and orientations of the major features of the plasmid are: lacZ' - EcoRI/MCS/HindIII lacZ' -> - pIP404 ori - <- rep - <- ermBP - pMB1 ori. [2] Growth: 1065 plus erythromycin (150 ug/ml) 37C Deposited by: Rood J.I.
Parents : ()
Siblings : ()
Descendents : ()

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