Vector IG Sequence Link :
General : plasmid ds-DNA 5400 BP
Functions : (cloning)(integrating)
Selection : ()
Copy Number :
Hosts : (E.coli)
Suppliers : (ATCC)
Misc.Comments : Suicide vector permitting gene replacement and mobilization into a wide range of Gram negative bacteria. Allows positive selection for integration. Replicates only in enterobacteria, and so functions as a suicide vector in other Gram negative hosts. When induced by media containing 5% sucrose, sacB is lethal in a wide range of Gram negative bacteria, and thus permits selection for loss of the vector. A single BglII site within the gentamicin resistance gene (gtmR) allows insertion of other resistance genes (e.g., as a BamHI cassette). The order of the major features of the plasmid is: gtmR - KpnI/MCS/SstI - P15A - traJ - oriT - sacB. [1] Deposited by: M.F.Hynes Grwoth media: LB plus gentamicin (ATCC medium number 1885) 37C
Parents : ()
Siblings : ()
Descendents : ()

Return to Vector Homepage