Vector IG Sequence Link :
General : plasmid ds-DNA 2762 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli)(E.coli HB101)(E.coli JM105)(E.coli JM109)
Suppliers : (ATCC)
Misc.Comments : Created by Moore, July 1995, under contract with NCBI. MCS. oligonucleotide linker. Deposited by: J. Justin McCormick Cloning vector with a second multiple cloning site inserted at the KasI site of pUC19 not interrupting lacZ'. [1] The plasmid contains the following restriction sites (bp from 0): NotI--0; Nsi--75; PvuI--119, 1907; EcoRI--237; HindIII--288; SapI--524; DrdI--749, 2618; BsaI--1607; SspI--2342. (personal communication) The order of the major features in this plasmid is: NotI/MCSII/NsiI - 3' lacZ' - EcoRI/MCSI/HindIII - 5' lacZ' - SapI - pMB1 ori - ampR. [1] Restriction digests of the clone give the following sizes (kb): PvuI--1.8, 1.0; EcoRI--2.8; HindIII--2.8; SspI--2.8; ClaI--2.8. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : (pUC19)
Siblings : ()
Descendents : (pMB)(pTB)

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