pK184


Vector IG Sequence Link :
General : plasmid ds-DNA 2432 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli XL1-Blue)(E.coli)
Suppliers : (ATCC)
Misc.Comments : A general purpose plasmid vector for use in complementation analysis with vectors having a ColE1 or pMB1 origin of replication. pK184 consists of bases 2092-2661/0-1177 of pK18 and bases 1520-836 of pACYC184. There are 2 XbaI sites in the plasmid. pK184 (ATCC 37766) and pK194 (ATCC 37767) have the multiple cloning sites in opposite orientations, interrupting the lacZalpha coding sequence. Constructed by M. G. Jobling. (personal communication) Restriction digests of the clone give the following sizes (kb): HindIII--2.4; BamHI--2.4; EcoRI--2.4; XbaI--1.45, 0.95. (ATCC staff) Medium is 1236 LB plus kanamycin.
Parents : ()
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Descendents : ()

NCBI ENTREZ Link :


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