pKB1020


Vector IG Sequence Link :
General : plasmid ds-DNA 14800 BP
Functions : (cloning)(integrating)
Selection : ()
Copy Number :
Hosts : (E.coli)(E.coli KB358)
Suppliers : (ATCC)(Omnigene, Inc.)
Misc.Comments : Restriction digests of the clone give the following sizes (kb): ClaI--14.5, 0.3; KpnI--14.8; BglII/KpnI--11.0, 3.5, 0.3. (ATCC staff) Host integration vector for adapting E. coli strains as hosts for att-Int mediated rearrangement and regulation of lambda-based expression vectors. Constructed by insertion of a lambda gene cassette into the bacteriophage Mu based vector pEG5005 (ATCC 67463), which is defective for the morphogenic functions. To maintain the plasmid, grow at 30C with ampicillin and kanamycin selection. Thermal induction (42C) of the Mu cts lysogen containing the plasmid yields a low titer phage lysate. Lysates should be used within 1 week of preparation since the clones can be unstable. Infection of sensitive, Mu cts lysogenic E. coli strains and growth at 30C yields two types of kanamycin-resistant isolates: ampicillin-sensitive strains (integrants) that carry no plasmid DNA, and ampicillin-resistant strains that contain the plasmid. [2] Note: This material is cited in a U.S and/or other Patent and may not be used to infringe the patent claims. Growth: LB plus amp (50ug/ml) plus kan (20ug/ml)(ATCC med num 1948)30C
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