Vector IG Sequence Link :
General : cosmid ds-DNA 9900 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(Aspergillus nidulans)(E.coli)
Suppliers : (ATCC)
Misc.Comments : The BamHI site was removed from trpC by sodium bisulfite mutagenesis. A 2.1 kb HindIII/SalI fragment (pJB8) containing lambda cos was ligated to HindIII/SalI-digested pBR329. A 4.1 kb XhoI fragment with wild-type Aspergillus nidulans trpC was ligated into the SalI, and a BamHI linker into the NruI site of pBR329. This is a cosmid vector for isolating genes by complementation of mutations in Aspergillus nidulans. It can also be used for library construction. Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()

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