pKL19-2


Vector IG Sequence Link :
General : plasmid ds-DNA 2675 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (depositor-supplied DNA)(E.coli)
Suppliers : (ATCC)
Misc.Comments : Created by Moore, July 1995, under contract with NCBI. Expression vector permitting production of a fusion protein and visual detection of recombinants, using the beta-galactosidase alpha peptide as the reporter group. (ATCC staff) A pUC19 derivative with 2 XmaI sites--one at nt 412 in the multiple cloning site and one added at nt 1567 by replacing DNA between the DraI sites at nt 1563 and 1582 with phosphorylated d[pCCCCGGGG]. (personal communication) Distributed in aliquots of 2 ug (100 ng/ul). (personal communication) Restriction digests of the clone give the following sizes (kb): PstI--2.7; BamHI--2.7; HindIII--2.7; EcoRI--2.7. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : (pUC19)
Siblings : ()
Descendents : ()


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