pKO-2


Vector IG Sequence Link :
General : plasmid ds-DNA 3755 BP
Functions : (promoter analysis)
Selection : (color red/white)
Copy Number :
Hosts : (E.coli GALK-)(bacteria GalE-T-K-)
Suppliers : ()
Misc.Comments : These data and their annotation were supplied to GenBank by Will Gilbert under the auspices of the GenBank Currator Program. Assembled from pKO1 and according to reference 1 by F. Pfeiffer. This is a promoter probe vector. The promoterless galK gene is used as selectable marker. Translationsal stop codons in all reading frames are present on a 11-bp oligonucleotide before the galK gene. Also, a polylinker was cloned before this gene. pKO2 contains a 4-bp deletion not present in pKM2. However, in GenBank SYNPKO2 and SYNPKM2 have been mixed up. Thus, SYNPKM2 is given in the Parent section.
Parents : (pKO-1)(EcoGalK)(pBR322)(pKM2)
Siblings : ()
Descendents : ()

NCBI ENTREZ Link :


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