pKT254omega-Ap


Vector IG Sequence Link :
General : plasmid ds-DNA 8900 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli C600)(broad host range)(E.coli)(Gram-negative bacteria)
Suppliers : (ATCC)
Misc.Comments : Restriction digests of the clone give the following sizes (kb): EcoRI--6.8, 2.7; HindIII--5.4, 2.6; (ATCC staff) An EcoRI fragment containing omega was ligated to EcoRI-digested pKT254. The spcR gene was deleted (SphI/BssHII double digest) and replaced with a 1.75 kb BamHI/BalI fragment of pKT235 containing the ampicillin resistance gene. [1] Because resistance levels may vary, it is important to determine the proper antibiotic concentration for each strain or isolate. [1] One of a series (ATCC 37653 through 37657) of vectors for in vitro mutagenesis (via the omega interposon) of gram-negative bacteria. [1] Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()


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