Vector IG Sequence Link :
General : plasmid ds-DNA 4049 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(E.coli)
Suppliers : (ATCC)
Misc.Comments : These data and their annotation were supplied to GenBank by Will Gilbert under the auspices of the GenBank Currator Program. Assembled according to the cloning strategy given in ref 1 by F. Pfeiffer. The STOP oligonucleotide TGATTGATTGA contains STOP codons in all three reading frames. It was inserted into pBR322 in different configurations: vector orien- pBR322 flank sequence flank pBR322 tation site site pKTH601 (forw) HindIII gc TGATTGATTGA cc BamHI pKTH604 ( rev) HindIII gc TCAATCAATCA cc BamHI pKTH605 (forw-rev) HindIII gc TGATTGATTGA-TCAATCAATCA cc BamHI pKTH606 (forw) EcoRI c TGATTGATTGA cc BamHI Vector containing portable translation stop codons in two oriendations (HindIII/stop/pots/BamHI). The depositor has requested that any proposed commercial use of the cells be negotiated with him. The sending of these cultures does not and should not be construed to grant any license or rights to the materials to the recipient. (ATCC staff) No distribution of the cultures or any derivatives therefrom should be made to third parties. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : (pBR322)(SFVE1STOP)
Siblings : (pKTH601)(pKTH604)
Descendents : ()


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