pL1


Vector IG Sequence Link :
General : plasmid ds-DNA 2586 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(vertebrate cells)(E.coli)
Suppliers : (ATCC)
Misc.Comments : Created by Moore, July 1995, under contract with NCBI. pcDV1 is an oligo dT-tailed plasmid primer. It is 3088 bp untailed. It works together with oligo dG-tailed pL1 linker, which has SV40 origin, SV40 early promoter, and SV40 mRNA splicing, and has a HindIII at one end and polyG at the PstI site at the other. pL1 linker has a BglI site in SV40 ori and an XhoI and BamHI site. A 16S splice junction is at the BamHI site. DNA ligase, RNaseH, and DNA polymerase I makes a dsDNA circle. cDNA synthesis is from polyA mRNA and is oriented, with linker 5' and polyA signal on plasmid 3' to cDNA. The SV40 segment contains KpnI and EcoRI sites for the preparation of vector-primer containing a poly(dT) tail at one end & the polyadenylation sequence from the late region of the virus. pcDV1 (ATCC 37368) and pL1 (ATCC 37369) should be used together to construct a cDNA library. Medium is 1227 LB plus ampicillin.
Parents : (pBR322)(SV40)
Siblings : ()
Descendents : ()


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