Vector IG Sequence Link :
General : cosmid ds-DNA 20000 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(broad host range)(E.coli)
Suppliers : (ATCC)
Misc.Comments : This vector transfers efficiently to a broad host range including E.coli HB101, Methylobacterium organophilum DSM 761, Pseudomonas AM1, Pseudomonas putida, and Pseudomonas aeruginosa. This was constructed from pLA2917 (ATCC 39840) by deleting the kanamycin resistance segment from Tn5 to avoid internal rearrangement when the transposon moves and to permit selection of conjugants that have a Tn5 insertion. This is a cosmid with a broad host range that can be used to characterize the genome of C1-utilizing microorganisms. Restriction digests of the clone give the following sizes (kb): PstI--20.0; HindIII--20.0; BglII--20.0; EcoRI--17.0; 2.6. (ATCC staff) Medium is 1273 LB plus tetracycline.
Parents : ()
Siblings : ()
Descendents : ()

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