pLD25


Vector IG Sequence Link :
General : plasmid ds-DNA 11000 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (Yarrowia lipolytica PC-30827 [ATCC 20688])(Yarrowia lipolytica)(E.coli)(E.coli JC-355)
Suppliers : (ATCC)
Misc.Comments : Constructed by a inserting a 6.6 kb Sau3AI fragment (partial) containing LEU2 into the BamHI site of pBR322. The gene is contained in an EcoRI fragment (partial) of approximately 2.4 kb. Replicates autonomously in E.coli and integrates but does not replicate autonomously in Y. lipolytica. The Y. lipolytica portion of the plasmid contains the following restriction sites: EcoRI (5); SphI (1); SalI (2); AvaI (8); KpnI (1); EcoRI (4); XhoI (2), BglII (2). It lacks HindIII, ClaI, BamHI, and NruI sites. Medium is 1245 YEPD. Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()


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