Vector IG Sequence Link :
General : plasmid ds-DNA 4100 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli)(E.coli WM1202)(E.coli JM103)(E.coli JM109)(E.coli LJ24)
Suppliers : (ATCC)
Misc.Comments : Deposited by: Walter Messer Vector containing the attP sequence for integrating DNA into the lambda attachment site attB. Requires the lambda integrase (int) such as that provided by pLDR8 (ATCC 77357). [1] When a recombinant construct is digested with NotI, the origin is separated from the insert. Each fragment has a distinct selectable marker. After religation to form origin-free circles, the products are transformed into bacteria with pLDR8. [1] Expression of int in pLDR8 is regulated by lambda PR, by the temperature sensitive cI857 repressor. Replication of pLDR8 is also temperature sensitive. [1] Therefore, at 30C replication of the plasmid is normal and int is not expressed. At 42C, int is expressed but pLDR8 is no longer replicated, resulting in loss of int after a few cell generations. [1] The order of the major features in this plasmid is: NotI - ampR - ClaI/MCS/EcoRI - attP - NotI - pMB1 ori - kanR. [1] Restriction digests of the clone give the following sizes (kb): EcoRI/BglII--3.7, 0.5; BamHI--4.2; XbaI--4.2; NotI--2.4, 1.8. (ATCC staff) Medium is 1227 LB plus ampicillin. E.coli LJ24 for integration
Parents : ()
Siblings : ()
Descendents : ()

Return to Vector Homepage