pLKC480


Vector IG Sequence Link :
General : plasmid ds-DNA 9900 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli TX528)(E.coli)
Suppliers : (ATCC)
Misc.Comments : The 6.3 kb lacZY-kanR cassette is released with SmaI. A 1.3 kb HindIII/NruI kanR fragment (with HindIII site filled in) was inserted at the NruI site of a lacZY fusion plasmid. One of a series of lacZY fusion vectors (ATCC 37594-37596) that allow fusions in one of three reading frames and retain flanking DNA for homologous recombination into the E.coli genome. Restriction digests of the clone give the following sizes (kb): EcoRI--10.05; BamHI--10.05; PstI--6.0, 4.4; HindIII--10.05. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : (pNEO)(pNM480)
Siblings : (pLKC481)(pLKC482)
Descendents : ()


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