pLKC481


Vector IG Sequence Link :
General : plasmid ds-DNA 9900 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli)
Suppliers : (ATCC)
Misc.Comments : One of a series of lacZY fusion vectors (ATCC 37594-37596) that allow fusions in one of three reading frames and contain flanking DNA for homologous recombination into the E.coli genome. A 1.3 kb HindIII/NruI kanR fragment (with HindIII site filled in) was inserted at the NruI site of a lacZY fusion plasmid. The 6.3 kb lacZY-kanR cassette is released with SmaI. Restriction digests of the clone give the following sizes (kb): EcoRI--10; SmaI--6.0, 3.7; BamHI--10; PstI--5.5, 4.4; HindIII--10. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()


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