pLS101


Vector IG Sequence Link :
General : plasmid ds-DNA 6400 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (Streptococcus pneumoniae R6)(broad host range)(E.coli)
Suppliers : (ATCC)
Misc.Comments : Cloning into the unique KpnI and SacI sites inactivates malM. Cloning into the 4.2 kb vector fragment resulting from HindIII digestion removes malM. Mal- recombinants can be enriched by penicillin selection of tetR bacteria. Cloning into the unique HpaII site inactivates tet. Removal of the smaller BclI fragment also inactivates tet. EcoRI digestion removes a dispensible EcoRI fragment. pLS101 contains the following restriction sites (kb markers according to the published map): PstI--0/6.4, 0.8; BclI--1.8, 2.8; HpaII--2.4; EcoRI--3.0, 4.1; HindIII--4.2, 4.9, 5.1, 5.3, 5.5, 6.2; KpnI--4.75; SacI--5.6. Strain is noncapsulated and reportedly never reverts to encapsulated form. This was constructed from pLS69 (ATCC 39937) by ligating a partial 2.0 kb HindIII fragment containing malM to a 4.4 kb HindIII from complete digest of pLS69. The latter fragment contains tet as well as the origin of replication. Medium is 1420 260 plus sucrose (0.2%) plus tetracycline (0.5 mcg/ml).
Parents : ()
Siblings : ()
Descendents : ()


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