pLS88


Vector IG Sequence Link :
General : plasmid ds-DNA 4772 BP
Functions : (cloning)(shuttle)
Selection : ()
Copy Number :
Hosts : (broad host range)(E.coli)
Suppliers : (ATCC)
Misc.Comments : Broad host range plasmid containing three antibiotic resistance markers. Natural isolate from Haemophilus ducreyi CH37. The sulfonamide resistance gene (sulII) contains PstI and HincII sites. The kanamycin resistance gene contains unique sites for ClaI, HindIII, PvuI, SmaI and XhoI. The order of the major features of the plasmid is: PstI - P6 promoter - sulII - strA --> - oriV - kanR --> - P5 promoter. [5] Demonstrated to transform (electroporation) Enterobacteriaceae, Pasteurellaceae, and Pseudomonaceae, but not Camplyobacter, Streptococcaceae, or Staphylococcus aureus (ATCC 25923). [3] Growth media: LB plus kanamycin (50 ug/ml) (ATCC number 1236) 37C Deposited by: W.L.Albritton
Parents : ()
Siblings : ()
Descendents : ()

NCBI ENTREZ Link :


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