pLSE4


Vector IG Sequence Link :
General : plasmid ds-DNA 6600 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli C600 [ATCC 23724])(broad host range)(E.coli)(Streptococcus oralis)(Streptococcus pneumoniae)
Suppliers : (ATCC)
Misc.Comments : The ScaI and EcoRV sites interrupt the tetracycline resistance gene. [1] The polylinker sites are upstream of the promoter-less lytA gene. There are stop codons in all 3 reading frames between the cloning sites and the initiation codon. [1] A HindIII fragment containing polylinker sequences from pUC19 and an XbaI/HindIII fragment containing the promoterless gene was cloned into the HindIII site of pLES1. The XbaI site was created 18 bp upstream of the initiation codon. [1] The sequence surrounding the polylinker is known so that inserts can be sequenced directly. [1] pLS1 and its derivatives can replicate in Streptococcus pneumoniae, S. oralis, Bacillus subtilis, and E.coli. [1] Contains the complete coding sequence for lytA. The 3' HindIII site is approximately 50 bp beyond the termination codon. [1] Medium is 1273 LB plus tetracycline.
Parents : ()
Siblings : ()
Descendents : ()


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