pLTRdhfr26


Vector IG Sequence Link :
General : plasmid ds-DNA 8600 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli RR1)(vertebrate cells)(E.coli)
Suppliers : (ATCC)
Misc.Comments : Restriction digests of the clone give the following sizes (kb): BamHI--8.6, 1.36; EcoRI--9.4; HindIII--9.4. (ATCC staff) A 3.3 kb EcoRI/BamHI fragment of HaSV clone H-1 (including the LTR) was cloned into EcoRI/BamHI-digested pBR322. A PstI fragment with mouse Dhfr (with BamHI linkers) was then cloned into the BamHI site of the construct. Transfected methotrexate-resistant NIH 3T3 cells carried double minute chromosomes that appeared to contain DNA acquired during transfection. Can be used in cotransfection experiments to generate a selectable methotrexate-resistant phenotype. Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()


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