Vector IG Sequence Link :
General : plasmid ds-DNA 4273 BP
Functions : (cloning)(expression up to 4000bp)
Selection : ()
Copy Number :
Hosts : (mouse)(E.coli HB101)(E.coli DH5alpha)(vertebrate cells)(E.coli)
Suppliers : (ATCC)
Misc.Comments : Expression shuttle vector with promoters for in vitro RNA synthesis. (personal communication) Consists of the following sequences: nt 1-51 from pGEM7Zf(+) (reverse of 28-78), 52-70 from pUC19 (reverse of 428-446), 71-1090 from pSV2neo (reverse of 3050-4069), 1091-3474 from pSP72 (88-2462 and 1-9), (personal communication) and 3475-4273 from pMP-1 (with 7505-8297 from MoMuLV). (personal communication) The order of the major features in this plasmid is: HindIII site of MCS (nt = 1) - SV40 splice acceptor - SV40 late polyadenylation signal - T7 promoter - bla - SP6 promoter - XhoI site - MoMuLV LTR. (personal communication) Note that the T7 and SP6 promoters are not immediately adjacent to the cloning sites. (personal communication) When cotransfected with pSV2-neo (ATCC 37149) into NIH 3T3 cells, 70-80% of G418-resistant colonies expressed high levels of appropriately sized mRNA. (personal communication) Restriction digests of the clone give the following sizes (kb): EcoRI--4.3; PstI--4.3; XhoI--3.6; 0.8. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : (pGEM-7Zf+)(pUC19)(pSV2neo)(pSP72)(pMP-1)
Siblings : ()
Descendents : ()


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