pLUC


Vector IG Sequence Link :
General : phagemid ds-DNA 5411 BP
Functions : (cloning)(promoter-cloning)(sequencing)
Selection : ()
Copy Number :
Hosts : (E.coli)(vertebrate)
Suppliers : (ATCC)
Misc.Comments : Promoter-cloning vector for analysis of eukaryotic promoters, using the luciferase reporter gene. Differs from pLUCS (ATCC 77415) only in the polylinker. Constructed from pSVOALdelta5' by inserting a 2.6 kb BamHI fragment, containing the firefly (Photinus pyralis) luciferase gene and SV40 sequences into the BamHI site of pBluescriptSK+, followed by the removal of the 3' BamHI site. Permits beta-galactosidase blue/white color selection for identification of recombinants. The color is weaker than from the parent vector and transformed cells may need to be grown 18-24 hours at 37C. The order of the major features in the phagemid is: 3' lacZ - XhoI/MCS/BamHI - 5' luc 3' - SV40 small t intron - SV40 polyadenylation signal - NotI - SstII - 5' lacZ - pMB1 ori - ampR - f1 ori. [1] Growth medium: LB plus ampicillin (ATCC medium number 1227) 37C Deposited by: A.D.Ward
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