pLUCS


Vector IG Sequence Link :
General : phagemid ds-DNA 5400 BP
Functions : (cloning)(promoter-cloning)(sequencing)
Selection : ()
Copy Number :
Hosts : (E.coli)(vertebrate)
Suppliers : (ATCC)
Misc.Comments : Promoter-cloning vector for analysis of eukaryotic promoters, using the luciferase reporter gene. Constructed from pLUC (ATCC 77414) by substitution of a StuI restriction site for the HindIII, PstI and BamHI sites in the polylinker. Permits beta-galactosidase blue/white color selection for identification of recombinants. The color is weaker than from the parent vector and transformed cells may need to be grown 18-24 hours at 37C. The order of the major features in the phagemid is: 3' lacZ - XhoI/MCS/StuI - 5' luc 3' - SV40 small t intron - SV40 polyadenylation - NotI - SstII - 5' lacZ - pMB1 ori - ampR - f1 ori. [1] Growth medium: LB plus ampicillin (ATCC medium number 1227) 37C Deposited by: A.D.Ward
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